The three lipid fractions (neutral lipids, glycolipids plus sphingolipids and phospholipids) were analyzed by TLC using various solvent systems.
Neutral lipids (N): A part of N fraction was separated using silica gel G-25 plate, developed with hexane/diethyl ether/glacial acetic acid (70:30:3, v/v/v). After development, the plate was dried under vacuum and was exposed to iodine vapor.
Glycolipids plus sphingolipids (G+S): A part of G+S fraction was separated using silica gel 60 F254 plate, developed with chloroform/methanol/water (85:15:2, v/v/v). G+S fraction chromatograms were visualized with
α-napthol (0.5 g α-napthol and 50 ml solution methanol:H2O/ 1:1). The plate was dried under vacuum and was sprayed with H2SO4 95% and was put at Τ=120°C γfor t=10 min.
Phospholipids (P): A part of P fraction was separated using silica gel 60 F254 plate, developed with chloroform/methanol/ammonia (28%) (65:25:5, v/v/v) and were detected with ninhydrin. (0,2% w/v to ethanol).